The spawning environment and seasonal changes in appearance of the Japanese silurid catfish Silurus tomodai were investigated in an upstream reach of a river in northern Mie Prefecture, central Japan. Eggs and larvae of the species were primarily found in highly localized areas around plant roots or in shallow accumulated litter at the water’s edge. Silurus tomodai was apparent from April to November, its activity increasing in the breeding season (from May to July). Laboratory feeding experiments indicated that juvenile proportions of lower jaw length to snout length, snout length to head length (HL), and interorbital width to HL may show greater variation than their mature counterparts.
Genetic characteristics of the Japanese torrent catfish Liobagrus reinii in the Miya River and neighboring drainages were investigated, using mtDNA and eight microsatellite (MS) markers, to evaluate the effects of man-made river structures on the distribution and genetic structure of the species. A total of 23 mtDNA haplotypes were detected, forming a star-like haplotype network, in which the population in the upper reaches (URM) formed a unique group. Many populations in tributaries of the middlelower reaches (MLRM) included unique haplotypes, although they shared a common haplotype located at the center of the network. MS markers indicated that genetic diversity tended to decrease upstream in the tributaries, coupled with a decline in effective population size and the existence of genetic bottlenecks. These phenomena were especially evident in tributaries isolated with weirs or dams. The fixation index RST, the values of which were smaller than FST, indicated isolation by distance (Mantel test), genetic differentiation among populations having occurred in recent years. Although a Bayesian-based assignment test showed unique clusters in the populations of isolated tributaries, including the URM population, many MLRM populations shared an admixture of multiple clusters, probably resulting from the dispersal of L. reinii. These results indicated that L. reinii in the Miya River included two conservation units, in the upper and middle-lower reaches, respectively. Man-made river structures seem to have caused fragmentation of the distribution of the species, resulting in small tributary populations suffering from genetic deterioration. In drainages neighboring the Miya River, the Isezi River population of L. reinii seems to be indigenous, owing to unique genetic characteristics in mtDNA and MS, whereas the sharing of genetic characteristics with the URM population of the Miya River indicated that the Akaba River population is likely to have been introduced from the Miyagawa Reservoir.
The triplefin genus Ceratobregma Holleman, 1987 includes two valid species, the Spotted Spiny-eye Triplefin C. acanthops (Whitley, 1964) and Helen’s Triplefin C. helenae Holleman, 1987. Both are characterized by the first dorsal fin having 3 spines, the anal fin 2 spines, and the pelvic fin with one spine and 2 rays, in addition to discontinuous lateral lines (upper series of tubular pored scales and lower series of notched scales), well developed lateral ethmoids, 3 or 4 spines on the anterior margin of the orbit in males, and a scaleless opercle and pectoral-fin base. Ceratobregma acanthops is known only from the Coral Sea, whereas C. helenae is widely distributed in the eastern Indian and western Pacific oceans. A single specimen (KAUM–I. 146836, 24.3 mm standard length) of C. helenae collected from Kume-jima Island, Okinawa Islands, Ryukyu Archipelago, Japan in October 2020 possessed the following characters: first spine of 1st dorsal-fin shorter than that of 2nd dorsal-fin; caudal peduncle narrow; sides of body with orange vertical bands; and 2nd dorsal fin with a basal series of orange spots. Previous records of the species from Japan include a single specimen (BPBM 8723, 29.6 mm standard length) collected from Taketomi-jima Island, Yaeyama Islands in 1968, and underwater photographs (as Ceratobregma sp.) taken at Irabu-jima and Miyako-jima Islands, Miyako Islands, in 2002 and 2004, respectively. The two Japanese specimens were examined, that from Kume-jima Island (described in detail) representing the northenmost record for the species. The new standard Japanese name “Mikan-hebigimpo” is proposed for C. helenae on the basis of the Kume-jima specimen, “Mikan-hebigimpo-zoku” serving for the genus Ceratobregma.
Two specimens of Callogobius tanegashimae (20.4 and 56.8 mm in standard length), collected from a muddy bottom in Wakasa Bay (bordering Fukui and Kyoto Prefectures), represent the first records of the species from the Sea of Japan. One specimen was collected with an alpheid shrimp using a yabby pump, suggesting that the goby may utilize shrimp burrows. The specimens are described in detail and their identification confirmed by reference to sequence variations on the mitochondrial DNA COI region (595 base pairs).
Mating behavior and early development of Cobitis sp. (BIWAE type D, TosaShima-dojo) were observed in the laboratory by inducing spawning of females with human chorionic gonadotropin (HCG). Subsequently, laboratory bred individuals were compared with spawned eggs and larvae obtained from the field. Mature adults, naturally spawned eggs, and larvae were collected from a river in eastern Kochi Prefecture, Shikoku Island, Japan, and a natural spawning ground adjacent to the river. Mating behavior was observed 15 times at night, and distinguished into four stages: phase 1, tracking; phase 2, approaching; phase 3, amplexus; and phase 4, spawning. Egg diameters after water absorption were 2.1–2.3 mm, the spherical, demersal eggs having a light-yellow yolk, no oil droplets, and slight viscosity. Newly hatched larvae [3.3–4.9 mm in total length (TL)] had 46 (32 + 14) myomeres, two pairs of outer gill filaments on the cheek, and melanophores on the head. Notochord flexion started at 6.0–7.2 mm TL (wild individuals at 6.0–6.3 mm TL) and was completed at 8.8 mm TL (6.6 mm TL). The formation of membranous fins and full fin-ray complements were attained in the fin order pectoral, caudal, dorsal, anal, and pelvic, and caudal, dorsal, anal, pectoral, and pelvic, respectively. Some individuals (7.0–8.0 mm TL at the flexion stage) had free neuromasts, each with a short cupula, laterally on the caudal region. In addition, following the postflexion stage, some morphological differences between artificially bred and wild individuals were observed but could not be quantified due to the small sample size.
The population structures of four Japanese species of the rajid genus Okamejei were investigated based on sequence variations in the mitochondrial DNA control region and morphometric data. These suggested that geographic barriers, such as straits and ocean currents, had shaped the population structures on a small spatial scale, the Tsushima Warm Current being particularly significant. This may be related to the limited migration ability of Okamajei species due to their small body size and habitat preference for shallow waters.
A population of freshwater Ayu (Plecoglossus altivelis altivelis) was found in the “wandos”, semi-enclosed remnants of artificial pond-like structures along the Yodo River at Shirokita, upstream of the Yodo River barrage. Microsatellite analysis of collected individuals showed their genetic origin to be a Lake Biwa strain, with a hatching date between September and November, estimated by otolith analysis. Otolith strontium and calcium concentrations indicated no history of seaward migration, the individuals analyzed having inhabited freshwater since hatching. In addition, eDNA analysis suggested that they had migrated from the main river course into the “wandos” in November.
Two specimens [FRLM 60553, 737.0 mm of total length (TL); SNFR 21750, 619.1 mm TL] of the moray eel Gymnothorax reevesii (Richardson, 1845) (new standard Japanese name “Mame-utsubo”) are reported from Shimane Prefecture (southwestern coast of Sea of Japan) and the East China Sea, respectively. Although the species has been reported as distributed in the South China Sea to Japan, in addition to Samoa and the Marquesas Islands, verification of the locality and identity of the two records from Japanese waters known to date are problematic. Accordingly, the specimens reported here are the first reliable, voucher supported records of G. reevecii from Japan, that from Shimane Prefecture being the northernmost record for the species.
The Indian mackerel Rastrelliger kanagurta is an important coastal fishery resource for Okinawa Prefecture, southwestern Japan. However, much is still unknown about its life history. The early development, occurrence, sexual maturation, and reproductive cycle of the species was examined from specimens collected from the coastal waters of Okinawa Island, and management of the fishery evaluated. Larvae [3.4 mm notochord length–12.5 mm standard length (SL)] and juveniles (11.4–16.2 mm SL) of R. kanagurta were distinguished from other mackerel species by: 1) numbers of myomeres, 2) absence of spines on the preopercle posterior margins, 3) positional relationship between the upper and lower jaw tips, 4) melanophore pattern, and 5) distribution (allopatric). Both larvae and juveniles occurred in the offshore epipelagic zone of Nakagusuku Bay in May, June, and August, which coincided with the occurrence of high-gonadosomatic value adults in coastal waters. However, specimens were not encountered in extremely shallow coastal areas (e.g., tidal flats), although younger individuals may utilize such the offshore epipelagic zone of the bay, attaining fork lengths (FL) of ca. 8 cm. Individuals mature at ca. 26 cm FL, one year after hatching. R. kanagurta are primarily caught by set net fishery near Okinawa, small (immature) individuals accounting for > 45% of netted individuals in all months, except May and June, during the period from April 1985 to April 1987, and for > 35% of the examined individuals in all months, except June and July, between April 2011 and March 2016. These results for both periods suggest growth overfishing. Accordingly, immature individuals must be conserved to sustain the Okinawan population of R. kanagurta.
Six specimens collected from Kochi, Nagasaki, Kagoshima, and Okinawa Prefectures, on the Pacific Coast and the East China Sea, and seven museum specimens, were identified as the purple ray Myliobatis hamlyni Ogilby, 1911, based on a combination of specific morphological characteristics. Initially considered an Australian endemic, subsequent studies have shown M. hamlyni to have a patchy distribution from the Australian coast to Japan, the latter record being based on a single specimen collected from Okinawa Prefecture. However, the current study has revealed the widespread distribution of M. hamlyni in waters from the surface to 500 m depth off southwestern and western Japan, a specimen from Kanagawa Prefecture representing the northernmost record of the species. Because the distribution of M. hamlyni may broadly overlaps that of the Japanese species M. tobijei, and morphometric characters and depth preference separating them suggested in the previous study were unclear, detailed comparisons were made so as to establish a basis for distinguishing between the two species in the present study. Myliobatis hamlyni differs most clearly from M. tobijei as follows: greatest span of pelvic fins 20.9–24.7% (mean 23.5%) of disc width [vs. 27.1–36.3% (30.7%) in M. tobijei], greatest span of pelvic fins 44.5–53.6% (50.4%) of pectoral-fin posterior margins [vs. 58.8–78.5% (67.4%)], and distance from edge of disc to first gill slit 51.0–68.3% (57.6%) of distance from pectoral-fin insertion to dorsal-fin origin (horizontal) [vs. 32.4–44.7% (41.3%)]. The new standard Japanese name “Sumire-tobiei” is proposed for M. hamlyni.
The Palemargin Grouper Epinephelus bontoides (Bleeker, 1855), previously recorded in Japan from Tanegashima, Yakushima, Kuchinoerabu, Amami-Oshima Island, and the west coast of Satsuma Peninsula, is newly reported from the southern part of Mie Prefecture, Honshu on the basis of a single specimen, which represents the northernmost record of the species as well as the first record from the main island of Japan. Possibly having been passively transported by the Kuroshio Current during its planktonic stage, the present specimen may have successfully overwintered due to heightened sea surface temperatures caused by the meandering current.
Two closely related, commercially important threadfin breams, Nemipterus furcosus and N. peronii, are an essential coastal fishery resource in Okinawa Prefecture. The age, growth, reproductive cycle, and stomach contents from 124 N. furcosus and 37 N. peronii, obtained from November 2011 to December 2015, were examined, age being assessed from sectioned otoliths and gonadal histology. Nemipterus furcosus and N. peronii were the most abundant threadfin breams in Kin Bay and Nakagusuku Bay, Okinawa Island, areas including many coastal tidal flats, where the two species comprised 98.1% of the total number of Nemipterus individuals examined. Overall sex ratios of both species were significantly sex-biased, the apparent lack of transitional gonads implying functional gonochorism. The spawning seasons of both species were estimated as occurring between spring and fall, no immature fishes having been obtained. Age validation using edge-type analyses implied that opaque zones were formed once per year, being valid annual growth increments. Although no intersex differences in maximum length, growth equation, and age range were observed in N. furcosus, N. peronii females were larger and older than males. The greatest ages observed were 4.3 and 7.0 years for N. furcosus and N. peronii, respectively. Both species fed predominantly on crabs, which primarily occupied the inner bays. Over the previous 27 years, the catch per unit effort of Nemipterus has declined in the highly altered environments of Kin and Nakagusuku Bays, suggesting that the decline in the populations of these species at Okinawa Island may be due to coastal fishery practices, environmental decline, and the degradation of suitable habitats. The biological implications for conservation are discussed.
Snailfishes (Cottoidei: Liparidae) are a large, morphologically diverse group of marine fishes, comprising about 32 genera with over 430 species worldwide. Among them, Osteodiscus Stein, 1978 is primarily distinguished from other genera in having a unique skeletal pelvic disk covered only by thin skin. Three species of the genus are currently known: Osteodiscus cascadiae Stein, 1978 from the eastern North Pacific; Osteodiscus andriashevi Pitruk and Fedorov, 1990 from southern Sea of Okhotsk; and Osteodiscus rhepostomias Stein, 2012 from southeast of New Zealand. During a taxonomic study of snailfishes, a single female specimen of Osteodiscus (150.3 mm in standard length) collected off Iwate in a depth of 1,997–2,108 m, was discovered in the fish collection of the National Museum of Nature and Science, Japan (NSMT). The specimen, characterized by 60 vertebrae (total), 54 dorsal- and 49 anal-fin rays, a horizontal mouth, simple blunt teeth on both jaws, some teeth with lateral cusps on the tip, an unnotched pectoral fin, and the presence of epipleural ribs and a reduced epural, was identified as O. andriashevi, previously known only from the holotype and three paratypes. The present specimen represents the first record of Osteodiscus from Japanese waters and the southernmost record of O. andriashevi. The new standard Japanese names “Hariban-kusauo-zoku” and “Choja-hariban-kusauo” are proposed for the genus and species, respectively. Based on the present specimen, the species diagnosis was partly revised.
The alepocephalid genus Conocara Goode and Bean, 1896 is characterized by the dorsal-fin origin located posterior to the anal-fin origin, dorsal-fin base shorter than the anal-fin base, body covered with small scales (> 80 in longitudinal row above the lateral line), tubular lateral line scales, the maxilla toothless, upper jaw equal to or longer than the snout, and photophores absent. Six specimens of Conocara werneri Nybelin, 1947, collected from Hyuga-nada Sea, Japan in a depth of 1,453–1,481 m, on 3 April 1991, are distinguished from all congeners by the following combination of characters: 17–20 dorsal-fin rays, 30–34 anal-fin rays, 159–179 longitudinal series scales above the lateral line, 19–22 scales between the dorsal fin insertion and lateral line, 25–32 scales between the anal fin insertion and lateral line, premaxillary bony crests present, the upper jaw reaching a vertical through the orbit anterior margin, an uninterrupted inner row of gill rakers on the first gill arch, the absence of palatine teeth, and raised insertions of the dorsal and anal fins with well-developed anterior cariniform skin folds. The gut contents of the six specimens represented the following higher taxa: Amphipoda, Copepoda, Ostracoda, Gastropoda (conch), Diatoma, Pyrosomata, and Foraminiferida. Four specimens possessed 113–550 developed ovarian eggs (maximum diameter 4.6 mm). Conocara werneri has been recorded previously only from subtropical zones of the eastern Atlantic and off New Zealand (south-western Pacific), the present specimens therefore representing the first record of the species from Japanese waters and northernmost record in the Pacific Ocean. The new standard Japanese name “Sedaka-yajiri-iwashi” is proposed for the species.
The threadfin bream genus Nemipterus Swainson, 1839 (Perciformes: Nemipteridae) currently includes 29 valid Indo-West Pacific species, eight of which have been recorded from Japanese waters. During an ichthyofaunal survey of Tanega-shima Island, Osumi Islands (Kagoshima Prefecture), Japan, a single specimen (246.9 mm standard length) of the Japanese Threadfin Bream Nemipterus japonicus (Bloch, 1791) was collected at a depth of 10 m on 13 January 2020. The specimen was characterized by seven anal-fin soft rays, 47 lateral-line scales, 6 + 10 = 16 gill rakers, a moderately deep body (depth 33.9% of standard length), long pectoral fin (posterior tip vertically level with anal-fin origin), posterior tip of depressed pelvic fin reaching between anus and anal-fin origin, upper lobe of caudal fin filamentous, and body pinkish dorsally and silver ventrally, with 1 longitudinal yellow stripes on the lateral surface, and a reddish blotch on the lateral line above the pectoral fin. Although the species is widely distributed in tropical and subtropical Indo-West Pacific waters from the Red Sea and the east coast of Africa to Taiwan and the Malay Archipelago, and bears the specific and Japanese names japonicus and Nihon-itoyori, respectively, it has at no time been recorded from Japanese waters. Therefore, the present specimen of N. japonicus, described here in detail, represents the first reliable record from Japan and northernmost record of the species in the western Pacific Ocean. The specimen collected from Tanega-shima Island was most likely to have been transported from Taiwan or the Philippines by the Kuroshio Current, the species being unlikely to reproduce in Japanese waters.