Genotyping of Fluconazole-resistant Candida albicans Isolated from Uighurian People in Xinjing (China) Using ALTS/RFLP and Micro-TGGE method

Abstract

Antifungal susceptibility tests were performed for 38 Candida albicans strains isolated from oral cavities of 43 Uighurian AIDS patients. Results showed that six isolates were resistant to fluconazole; one showed low susceptibility. We attempted to examine these strains molecular-epidemiologically, but 25S rDNA genotyping was insufficient for their discrimination. To estimate whether the origins of resistant strains were identical, we developed a new combination method of C. albicans tandem repeating units (ALTS)/RFLP and micro-temperature gradient gel electrophoresis (μ-TGGE). This new method was able to distinguish all seven strains. A suspected nosocomial infection was ruled out.