Abstract
The influence of various factors on minimum inhibitory concentration (MIC) and minimum cidal concentration (MCC) of miconazole (MCZ) on the growth of various pathogenic fungi was investigated.
Prolongation of incubation time hardly showed a significant influence on the MIC and MCC values of MCZ against both C. albicans and dermatophytes, viz. some species of Trichophyton, Epidermophyton, and Microsporum.
Increase in inoculum size affected cosiderably the MIC values against C. albicans yeast form cells and slightly against T. mentagrophytes conidia.
The MIC and MCC values were raised in alkaline pH environment.
The addition of whole blood, plasma, erythrocyte or serum raised both values. Calf serum also raised both values significantly, whereas the effect of bovine serum albumin was slight. Lipids extracted from calf serum decreased anti-C. albicans activity of MCZ remarkably.
