Abstract
Effects of fluconazole, bis-triazole containing antifungal agent, on sterol 14-C demethylation have been investigated for the whole cell and the cell free system of Candida albicans and also for the rat liver cell free system. In the cell free system, sterol 14-C demethylation was much more sensitive to fluconazole with C. albicans than with rat liver. Furthermore, the difference in binding affinity of fluoconazole to cytochrome p-450s of C. albicans and rat liver was observed to be well correlated with a greater inhibition by fluconazole for ergosterol biosynthesis in C. albicans cells as compared with cholesterol biosynthesis in rat liver.
