Abstract
A simple resazurin-coupled fluoroassay for serum D-arabinitol is described. This method involves two coupled enzymatic reactions, D-arabinitol dehydrogenase and diaphorase. The increase of fluorescence produced by the reduction of resazurin was determined with an excitation wavelength of 560nm and an emission wavelength of 580nm.
We serially determined serum D-arabinitol in a patient with acute lymphoblastic leukemia complicated with multiple Candida liver abscesses. When numerous small low density areas were recognized in the liver and the spleen by CT, serum D-arabinitol concentrations and D-arabinitol/creatinine ratios were found to be significantly increased, 10-20 fold above normal.
