Abstract
Monoclonal antibodies were raised against Aspergillus fumigatus, a fungus which is an etiological agent of aspergillosis. Male SPF BALB/c mice were immunized subcutaneously with A. fumigatus culture-filtrate or mycelial antigen. Their splenocytes were hybridized with mouse myeloma cells, P3 X 63 Ag 8.653 The hybridoma supernatants were screened by enzyme-linked immunosorbent assay (ELISA), using A. fumigatus culture-filtrate antigen. Of the 123 hybridoma clones, 27 cell lines secreting antibodies positive for A. fumigatus were obtained. Two of these cell lines were further grown to larger volumes. The monoclonal antibodies obtained were immunoglobulin-M (IgM) antibodies, which were then purified by ammonium sulphate precipitation and Mono Q anion exchange chromatography. The purified monoclonal antibodies showed reactivity against the protein constituent of the culture-filtrate antigen.
