Detection of Asp-hemolysin from Clinical Isolated Strains of Aspergillus fumigatus

Abstract

To detect Asp-hemolysin and its mRNA from clinical isolated strains of Aspergillus fumigatus (A. fumigatus), seventeen strains of A. fumigatus including A. fumigatus Fresenius-Muramatsu (FM) as a control were analyzed by Western and Northern blotting procedures. These strains were grown in liquid 199 medium and Sabouraud glucose broth medium. In the Western blot analysis, Asp-hemolysin was detected from the culture filtrate fractions of 12 isolates of A. fumigatus with liquid 199 medium. Northern analysis confirmed Asp-hemolysin mRNA of 0.7kb in 13 isolates of A. fumigatus with liquid 199 medium. These results indicate that, depending on the levels of protein or mRNA, several clinical isolates of A. fumigatus also produce Asp-hemolysin.