Abstract
Purpose: A method for determining the acoustic parameters of thin biological tissues using an acoustic microscope is proposed, taking into account multiple reflection. Subjects and Methods: In the method, the acoustic parameters of velocity, attenuation, and acoustic impedance are determined using measured phase and amplitude at around 10 different frequencies. Conclusion: The results of numerical calculations for simulation data including ±5% random errors show that the determination precision of the velocity, attenuation, and acoustic impedance are ±0.04%, ±2.7%, and ±1.2%, respectively.