Abstract
Various methods for extracting DNA from single nematodes for PCR amplification have been developed by many authors. Such techniques generally do not include a purification step, and direct application of crude lysate to PCR amplification is usual. SDS is not used in DNA extraction methods for PCR amplification from single nematodes, although it is a common detergent used in DNA extraction. This is because SDS has an inhibitory effect on PCR (Gelfand, 1989). The author developed an SDS-based DNA extraction method from single nematodes, where the crude lysate can be applied directly to PCR amplification, and reports it here.
