Simple methods for morphological observation of nematodes

Abstract

Some quick and easy techniques for nematode morphological observations are provided. Silicone grease or petroleum jelly can be applied to adjust the nematode direction to observe lateral or ventral aspects. Small spots of the grease are placed between the slide glass and coverslip to fix the coverslip and adjust sample thickness. Nematodes can be turned by moving the coverslip, which can be pushed to observe the complicated stomatal morphology. Squashing of the nematode is handy to evert the buccal cavity. Furthermore, diluted detergents (0.1-0.5% Tween 20 or 80) can be used to remove bacterial particles stuck on the nematode surface and observe the surface structure of the nematode.