Protocol for root-knot nematode culture by a hydroponic system and nematode inoculation to Arabidopsis

Abstract

Root-knot nematodes (Meloidogyne incognita) are one of the most economically damaging nematodes; thus it is important to know the molecular mechanisms involved in the phases of nematode infection. To use this nematode as a material for research, we must constantly prepare sufficient numbers of nematodes, preferably sterilized. Here we have developed alternative methods for culture, sterilization, and inoculation of root-knot nematodes that are especially suitable for the use of Arabidopsis thaliana Heynh as host plants.