Nematological Research (Japanese Journal of Nematology)
Online ISSN : 1882-3408
Print ISSN : 0919-6765
ISSN-L : 0919-6765
RESEARCH NOTES
Protocol for root-knot nematode culture by a hydroponic system and nematode inoculation to Arabidopsis
Hidetaka NishiyamaBui Thi NganSatoru NakagamiChica EjimaTakashi IshidaShinichiro Sawa
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2015 Volume 45 Issue 1 Pages 45-49

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Abstract

Root-knot nematodes (Meloidogyne incognita) are one of the most economically damaging nematodes; thus it is important to know the molecular mechanisms involved in the phases of nematode infection. To use this nematode as a material for research, we must constantly prepare sufficient numbers of nematodes, preferably sterilized. Here we have developed alternative methods for culture, sterilization, and inoculation of root-knot nematodes that are especially suitable for the use of Arabidopsis thaliana Heynh as host plants.

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© 2015 The Japanese Nematological Society
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