Abstract
In an attempt to elucidate toxin production in pine seedlings by the pine wood nematode, Bursaphelenchus xylophilus, and an associated bacterium, young seedlings of Pinus thunbergii which had formed callus tissues on root tips were inoculated with aseptic nematodes or bacterium-contaminated nematodes in test tubes. In the former most of inoculated seedlings collapsed by the nematode invasion. Several seedlings remained healthy in appearance although the nematodes propagated on root callus and/or invaded the stems.
Bacterium-contaminated nematodes did not affect the seedlings.
Culture filtrate of three bacterial isolates from the dispersal forth-stage larvae of the nematode showed toxicity to young seedlings, but the cell suspension of an isolate, of which culture filtrate showed the strongest toxicity, did not affect three-year-old Pinus densiflora seedlings while aseptic nematodes killed them. Jpn. J. Nernatol. 13:1-5(1983)
