Propagation of Bursaphelenchus xylophilus on Callus Tissue of Jeffrey Pine, Resistant to Pine Wilt

Abstract

The pine wood nematode, Bursaphelenchus xylophilus, was found to reproduce on callus tissues of Pinus densiflora and P. thunbergii which are susceptible to pine wilt caused by this nematode and on callus tissues of lucern and cryptomeria which are not the hosts of this nematode. In the inoculation test in the United States, P. jeffreyi showed low susceptibility. The objectives of the present study were to determine the propagation of the nematode on callus tissues of P. jeffreyi compared with those of P. densiflora and P. thunbergii and to examine the difference between callus tissues and ordinary seedlings of P. jeffreyi in the propagation of this nematode.