Thermal Effects on the Fine Structure of the 2nd Stage Larvae of Heterodera glycines Ichinohe
1976 Volume 6 Pages 27-34
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1976 Volume 6 Pages 27-34
The structural changes of the 2nd stage larvae of Heterodera glycines, which were exposed to temperatures at-20°C and 40-60°C, were investigated under light and electron microscopes. Normally the cuticle was 0.64 μm in thickness and composed of 4 layers. The thickness of cuticle changed slightly by thermal treatments and the ratio of cuticle to body diameter decreased significantly by the treatment at-20°C for 1 hr. Spacial arrangements of myofilaments were disordered in the nematodes treated at 60°C, while they were stable at 45°C for 5 min and at-20°C for 60 min. Lipid droplets became irregular in shape at 60°C. Mitochondria and glycogen were disordered by all thermal treatments. Jap. J. Nematol. 6:27-34(1976)
