Abstract
For identifying Pratylencus penetrans, species-specific primers (PP1 and PP2) were developed, based on the sequences of the interscribed spacer regions (ITS1 and ITS2) of the rDNA. When the purified DNA from seven Pratylenchus species was subjected to PCR using these primers, the 462 bp fragment was generated only for P. penetrans. The same fragment was obtained with DNA from the crude lysate of single juvenile and of adult (male and female) P. penetrans.
