Mass extraction method for determining Aphelenchoides besseyi density in Oryza sativa seeds

Abstract

The efficiency of a mass extraction method is described for obtaining the white tip nematode, Aphelenchoides besseyi Christie, at low densities from batches of 20, 50, 100, or 150 seeds of rice, Oryza sativa L. In the method, individual rice seeds were split longitudinally and then placed on a stainless wire netting tray. The tray containing spilt seeds was placed in a container with water to soak the seeds for 4 hours at 25°. The proportion of extracted nematodes reached almost or completely 100% irrespective of the batch size of seeds. Consequently, the mass extraction method was applicable for determining the low nematode densities per seed and more efficient than the time-consuming, single seed method of Hoshino and Togashi.