Effects of Iodine Tincture Application and Cepharanthin^® Intraperitoneal Administration on Rat Oral Mucosa

Study by Histology and PCNA Staining Method

Abstract

In this study, the effects of iodine tincture application and cepharanthin^®intraperitoneal administration on oral mucosa of the rat were investigated, by both histological and PCNA immunohistochemical staining methods. In the iodine tincture application group, the gingiva and buccal mucosa exhibited mild to moderate changes of epithelial dysplasia that were not observed in the control group. These epithelial changes included irregular arrangement and cellular atypia of basal cells, irregular arrangement and proliferation of prickle cells, thickening of the keratinized cell layer, and irregular elongation of epithelial projections. The PCNA percentages in the gingiva and buccal mucosa increasedl significantly compared with the control group. In the cepharanthin intraperitoneal administration group, gingiva and buccal mucosa were similar in histology and PCNA percentages to those of the control group. In the iodine tincture application + cepharanthin intraperitoneal administration group, the gingiva and buccal mucosa displayed mild changes of epithelial displasia that were not seen in the control group. The PCNA percentages in the gingiva and buccal mucosa were statistically significant compared with those of the control group. These changes were less marked than those of the iodine tincture application group.