1987 Volume 33 Issue 11 Pages 2081-2090
Fibrin membranes, collagen membranes and gelatin capsules were used as rapping materials to prevent the diffusion of the implanted powder-like decalcified bone matrix. The powder-like decalcified bone matrix combined with rapping materials were implanted under the fascia of the abdominal muscle of rats to observe the effect on new bone formation by the rapping materials which were absorbed in the rat during different periods. In addition the histological changes of the decalcified bone matrix due to the response of the rat were also observed.
The results were as follows.
1. Implantation of the decalcified bone matrix alone showed the best bone formation ability, and the new bone formation was not promoted by using rapping materials.
2. Neither new bone formation nor calcification were observed in the groups sacrificed at 3 weeks and 5 weeks after the operation, but only a residue of the decalcified bone matrix were observed. This means that the bone formation ability was lost during these periods. Consequently, it is clear that if the decalcified bone matrix is kept in vivo for long time, the promotion of new bone formation does not occur.
3. When the implanted area suffers from the inflammation, the decalcified bone matrix was calcified again, but the new bone formation was not occurred.
4. The effect of the rapping materials was not outstanding, but it was considered that atelocollagen had the highest possibility for clinical application among the rapping materials which were experimented with in this study. It was felt that this would be even more so when combined with the purified Bone Morphogenetic Protein to make up the appropriate shape for implantation.