Induction of cleft palate in ddY mice by staphylococcus aureus inflammation of mouth floor

Abstract

Great importance has been attached to environmental factors as causes of abnormality; several studies have been reported.
The author examined the occurrence of abnormality due to infection-induced inflammation.
Materials and methods were as follows: ddY mice were used. The first day of pregnancy was considered to be the day a plug was found. Some pregnant females were given injections of Staphylococcus aureus suspention in the dorsal subcutaneous tissue and the others in the fl oor of the mouth 11th day of gestation. Animals were sacrificed on the 16th to 17th day of gestation.
The implantations, dead fetuses and the presence of cleft palate were noted.
The results obtained were as follows:
1. In the group infected with staphylococcus aureus in the dorsal subcutaneous tissue, incidence of cleft palate was 1. 92% on the 10 th day, 0.64% on the 11 th day, and 0% on the 12 th day of pregnancy.
2. In the group injected with saline in the dorsal subcutaneous tissue, incidence of cleft palate was 0% on the 10 th, 11 th, and 12 th day of pregnancy.
3. In the group infected with staphylococcus aureus in the floor of the mouth, incidence of cleft palate was 4.35% on 10 th, 10.0% on 11th, and 8 74% on 12th day of pregnancy.
4. In the group injected with saline in the oral floor, incidence of cleft palate was 0% on the 10 th, 11 th, and 12th day of pregnancy.
5. From the above results, it is suggested that inflammation due to infection is a possible cause of cleft palate.