1992 Volume 38 Issue 1 Pages 48-52
Ampicillin (ABPC) is widely used prophylactically and in the treatment of infection. When ABPC is administered for prophylaxis of postoperative infection, the target tissue is subjected to a surgical damage. Since the damaged tissue by surgery is different from the normal tissue, the distribution of ABPC might be affected. Thus, the present investigation was undertaken to compare between the ABPC concentrations in damaged tissue and normal tissue, using incised skin asa damaged tissue and untreated skin asa normal tissue. Pharmacokinetic analysis was also performed to show numerical differences of ABPC concentrations between incised and untreated skins.
Wistar strain SPF male rats, 12-weeks old, were used. Ampicillin·Na (ABPC·Na, 100mg/kg) was administered intravenously. Rat head skin was incised immediately after administration of ABPC·Na.Specimens of incised skin (surgical tissue) and untreated skin (normal tissue), were collected at O.25, O.50, O.75, 1.0, 1.5, 2.0, 3.0, 4.0, 6.0 and 8.0 hours after administration of ABPC·Na.Blood samples were also taken at the those times. ABPC concentrations in serum and the skins were assayed by a paper disk method. The pharmacokinetic analysis was performed to compare the ABPC concentrations in serum and incised and untreated skins.
The results are as follows:
1. The concentration of ABPC in serum at O.25 hours after administration was 132.20±14.33μg/ml. ABPC level was rapidly deceased during O.25 to 1.0 hours after administraion, and then gradually decreased to reach 0.09±0.07μg/ml at 8.0 hours.
2. ABPC concentrations in all incised skins were continually higher than those of the corresponding untreated skins (t-test, p<0.10).
3.K13/V and K31 of incised skin weresmaller than those of untreated skin, and AUC of incised skin was larger. Reasonable differences between incised and untreated skins could be also shown by the pharmacokinetic parameters.
