1997 Volume 43 Issue 2 Pages 55-61
The authors attempted to develop a new cancer chemotherapy with magnetoliposomes containing CDDP for the treatment of head and neck squamous cell carcinoma. First, preparations of magnetoliposomes containing CDDP and the effects of these liposomes on cultured tumor cells were examined. Magnet particles were purified from Magnetspillium sp. AMB-1. These particles and CDDP were incorporated into liposomes prepared with phosphatidylcholine and cholesterol. The antitumor activity of the magnetoliposomes against mouse squamous cell carciroma (KLN250 cells) was evaluated by an XTT assay. Magnetoliposomes containing CDDP showed distinct cytotoxic activity against KLN205 cell at the concentration of 5 μg CDDP/ml. At this concentration of CDDP, there was no cytotoxic activity of CDDP alone. The results suggest that magnetoliposomes containing CDDP may be an effective drug delivery system for cancer treatment. Magnetoliposomes can remain around tumor cells for prolonged periods by application of an external magnet field, and CDDP can be released slowly and continuously.
