Phenytoin and Its Metabolite, 5-(4-Hydroxyphenyl)-5-Phenylhydantoin, Show Bone Resorption in Cultured Neonatal Mouse Calvaria

Abstract

The effects of phenytoin and its major metabolite, 5−(4−hydroxyphenyl)−5−phenylhydantoin(HPPH), on bone resorption of neonatal mouse calvaria were examined in vitro.Both phenytoin and HPPH induced significant bone resorption as compared to the controls after 72 h in culture.This effect may be the cause of phenytoin−induced bone loss in vivo.