1997 Volume 11 Issue 6 Pages 414-419
We previously reported that alloantigen-specific cytotoxic T lymphocytes (CTL) could be induced similarly in both cord and adult blood mononuclear cells. Here, we report the kinetics of IL-2 production of cord blood mononuclear cells and the effect of immunosuppressants on IL-2 production. A mixed lymphocyte culture was set up, using cord blood mononuclear cells as responders and irradiated allogeneic mononuclear cells of a healthy adult as stimulators. IL-2 concentration was serially determined from day 1 to day 6. There was no significant difference in IL-2 production in cord and adult blood mononuclear cells. Immunosuppressants, cyclosporin A and FK506, significantly inhibited IL-2 production. These in vitro results, together with our previous observation, suggest that IL-2-dependent CTL may cause graft-versus-host disease and also graft-versusleukemia effect in cord blood stem cell transplantation.
