Abstract
Morphological observation of blood and bone marrow films with light microscope is a fundamental and essential part of hematology. Light microscopy is observing the light coming through the specimen. Therefore, satisfactory observation requires well-prepared specimens of good quality and appropriate illumination. The bone marrow specimen of good quality must contain bone marrow spicules. The preparation of good quality must be made according to the method traditional and familiar to many hematologists and have a large observable area where red blood cells are evenly spread without overlapping and have discoid shape with central pallor. The light of good quality can be obtained with the Köhler illumination method. In order to obtain a bright picture with good contrast, the diameters of the field iris diaphragm, the aperture iris diaphragm and location of the condenser must be appropriately adjusted.
