Productive Ability of Granulocyte/Macrophage-Colony Stimulating Activity (GM-CSA) by Monocytes Obtained from Children

Undergoing Peripheral Blood Stem Cell Autografts

Abstract

Productive abilities of granulocyte/macrophage-colony stimulating activity (GM-CSA) by monocytes were evaluated in children undergoing peripheral blood stem cell autograft. For this purpose, monocytes obtained from the blood before apheresis, collected cells in the apheresis bag and thawed grafts were cultured with or without M-CSF and then, supernatants were tested for GM-CSA in methylcellulose colony assay system with cord blood cells as a target population. The results indicate that monocytes were activated de novo at the time of harvesting and productive ability of GM-CSA had been retained during freeze-thaw procedure. There is a probability that hematopoietic growth factors produced by these activated monocytes play a part in the early recovery of hematopoiesis observed after peripheral blood stem cell autografts.