Abstract
Glycyrrhizin was studied by thin layer chromatography (TLC) using an NH2-chemically bonded stationary phase, prepared from pre-coated silica gel plate treated with benzene solution containing 3-aminopropyltriethoxysilane (3APTS). TLC plate treated with 3APTS were applied to the'evaluation of glycyrrhizin in commercial drug samples. The 3APTS treated plates were developed in a chromatographic chamber at room temperature with n-BuOH/ NH4OH/ EtOH (5: 2: 1) to a distance of 8 cm from the origin. The plates were dried, then treated for detection. Glycyrrhizin appeared as a dark violet spot on a blue background in UV light.
The UV absorbance of the glycyrrhizin spot on the 3APTS treated plate was measured at 254 nm with a Shimadzu dual-wavelength TLC Scanner CS-910, and the quantitative determination was carried out with a Shimadzu C-R1A Chromatopac.
