Abstract
In an attempt to evaluate the reliability of apoenzyme reactivation immunoassay system (ARIS) method, phenobarbital (PB) and phenytoin (PHT) concentrations in patient's serum were measured by ARIS method, and were compared with those obtained by fluorescence polarization immunoassay (FPIA) method and enzyme multiplied immunoassay (EMIT) method.
The within-run precision of ARIS method was 2.56-5.26% as the coefficient of variation (C. V.). The C. V. value of between-run precision was less than 6%. There were significant correlations between the serum concentrations determined by ARIS method and each method (FPIA; EMIT). The interference from hemoglobin, bilirubin, ascorbic acid, flavin adenine dinucleotide (FAD) and cross-reacting substances was also investigated and discussed.
ARIS method was found to be reliable, simple and rapid one. Therefore, ARIS method seemed to be a useful technique for the rapid determination of drug concentration.
