Inhibitory Effects of Various Protease Inhibitors on Complement-mediated Hemolysis

Abstract

Inhibitory effects of various protease inhibitors on complement-hemolysis were investgated in vitro.In the hemolytic action system for classical pathway, sensitized sheep erythrocytes at a concentration of 2.0×10⁸ cells/0.2ml and diluted normal human serum were used, and in alternative pathway, unsensitized rabbit erythrocytes at a concentration of 1×10⁸ cells/0.4ml and diluted normal human serum were used.
Gabexate mesilate, camostat mesilate and nafamostat mesilate inhibited complement-hemolysis including both the classical and alternative pathways, but aprotinin and urinastatin did not inhibited at 100 fold concentration of usual dose.Among these protease inhibitors, nafamostat mesilate strongly inhibited complement-hemolysis.With usual dose, nafamostat mesilate obviously indicated anti-complement activity.