Abstract
A study was made to find out how admixtures of syrups of Alimezine, Inolin and Leftose become discolored during storage for 5.5 hr at room temperature. As for the discoloration of Alimezine syrup from red (λmax510nm) to yellow (λmax445nm), the change of absorbance with new coccine in the Alimezine syrup was read at 510 nm with a spectrophotometer. The degree of discoloration of Alimezine syrup in the admixture depended on the concentration of syrups of Inolin and Leftose. Sodium sulfite in the Inolin syrup was essential for discoloration in the admixture. The discoloration in the admixture was accelerated by the lysozyme chloride contained in Leftose syrup. This discoloration was reconstituted with Alimezine syrup, Inolin syrup and a substitute for Leftose in which lysozyme chloride was replaced by bovine serum albumin. In the admixture of the syrups of Alimezine, lnolin and Leftose, new coccine and the yellow discoloration product (yellow new coccine) were bound to lysozyme chloride. These results suggest that the discoloration of new coccine in the admixture of the three kinds of syrups is produced by sodium sulfite and the binding of new coccine to lysozyme chloride.
