Abstract
Amylase isozymes in commercial multiple digestive enzyme preparations were detected by a discontinuous buffer system in which Tris-Borate buffer was used for supporting medium and Veronal buffer for electrophoretic chamber. The supporting medium was first immersed in Tris-Borate buffer, then electrophoresis was performed with Veronal buffer. Oxoid membrane of cellulose acetate was used, and Dy Amyl (warner-Lambert) and Blue Starch (Pharmacia) were employed as substrates. In this procedure, the isozyme bands were well separated. Quality index of 11 enzyme preparations was made up on the basis of total amylase activity determined after incubation for 2 hours at 37° or 56°. The quality index corresponded to isozyme pattern.
