Abstract
Effect of parenteral amino acid solution on erythrocytes was examined on the basis of changes in osmotic fragility of erythrocytes with a coil planet centrifuge. At the same time, morphological changes in erythrocytes were examined by a scanning electron microscope, and changes in particle distribution of erythrocytes by automatic particle counter (HIAC). Effect of parenteral amino acid solution on erythrocytes became greater in parallel with the increase in osmotic pressure of the solution, and this was confirmed by the result of scanning electron microscopy. Even when a hypertonic amino acid solution was diluted to the isotonic solution, osmotic fragility of erythrocytes was varied depending on the kind and quantity of amino acids present and the kind of sugars added. The fragility was also affected by mixing ratios of blood and amino acid solution. Sodium hydrogen sulfite added to the parenteral solution as an antioxidant was found to have no specific effect on the osmotic fragility of erythrocytes. These results indicated that the test conditions should be taken into consideration when the effect of drugs on erythrocytes is judged.
