Abstract
Quality of high-molecular-weight substances was tested by thin-layer gel filtration chromatography (TLGC). Preparations selected for the tests were those of lysozyme chloride, urokinase, serratiopeptidase, cytochrome c, trypsin/α-chymotrypsin, trypsin/bromelain, streptokinase/streptodornase, and insulin. Pharmacia TLGC apparatus was used for the analysis. A 0.025M phosphate buffer system (pH7.4) containing 1.5% sodium chloride was mainly used as developing solvent, and nigrosine as color developing agent. A good reproducibility was demonstrated in the tests by the TLGC. It was found that the TLGC, simple and convenient in operation, is a reliable method of analysis for the quality control of these preparations.
