Abstract
The insolubilized antibodyc-enzyme immunoassay technique (MARKIT method) was studied in terms of its clinical application in determination of carbamazepine (CBZ) and valproic acid (VPA) in serum. Each coefficient of variation for the three levels of CBZ or VPA was less than 8% within-run and between-run. The serum CBZ concentration determined by the MARKIT method was compared with that determined by high-performance liquid chromatography (HPLC) or the homogeneous enzyme immunoassay technique (Emit method). There was good agreement between the MARKIT method and HPLC results, and the MAKRIT method and the Emit method results, where the correlation coefficient was 0.999 and 0.998. The metabolite of CBZ, carbamazepine-10, 11-epoxide, did not interfere with the MAKRIT method under therapeutic conditions. The serum VPA concentration determined by the MARKIT method was compared with that determined by gas liquid chromatography (GLC) or the Emit method. There was good agreement between the MARKIT method and GLC results, and the MARKIT method and the Emit method results, where the correlation coefficient was 0.996 and 0.997.
Consequently the MARKIT method will be usefulfor the serum level monitoring to patients undergoing CBZ and VPA therapy.
