Abstract
A comparative study of the antioxidant effects of Japanese Kampo medicines based on the tonic effects of Hochuuekkitou (EK41) and Ninjinyoueitou (EK108) were estimated by measuring the SOD like activity, linoleic acid oxidation rate and antioxidant capacity.
As a result, the SOD like activities of EK41 and EK108 were investigated, EK41 and EK108 showed a 50% inhibition at concentrations of 0.39mg/mL and 0.34mg/mL, respectively, according to the nitrate method, while EK41 and EK108 also showed a 50% inhibition at concentrations of 0.02mg/mL and 0.06mg/mL, respectively, according to the nitrobluetetrazolium method.
The oxidation rate of linoleic acid by FeSO4 doubled at a final concentration of 0.315mg/mL in EK41 and EK108.
However, these rates of linoleic acid by AMVN were not affected after the addition of EK41 and EK108.
The antioxidant capacities of EK41 and EK108 were equivalent to 0.65mM and 0.98mM of the capacity of Trolox C, respectively, at a concentration of 10mg/mL.
The antioxidant activity of both EK41 and EK108 were thus found to be similar to those previously reported using different assay methods.
