Novel Inverted Triple Grease-Gap Isolation Chamber for Electrophysiological and Calcium Release Studies in Skeletal Muscle Fibers

Abstract

A novel inverted triple grease-gap chamber for electrophysiological studies and photometric detection of calcium release in skeletal muscle fibers is described. The chamber allows the construction of grease seals displaying high electrical resistance and mechanical stability. The fiber mounting procedure is fast, simple and not skill demanding, and the preparation is highly stable and reliable. Electrophysiological and photometric data are presented to illustrate the chamber performance.