Selective Recording of Electroneurograms from the Sciatic Nerve of a Dog with Multi-Electrode Spiral Cuffs

Abstract

Electroneurograms (ENGs) from superficial regions of the sciatic nerve of a Beagle dog were recorded selectively with a chronically implanted 33-electrode spiral cuff (cuff). By delivering stimulating pulses to groups of three electrodes (GTEs) within the cuff we could define the relative positions of the particular superficial regions that selectively innervated the tibialis anterior (TA) and gastrocnemius muscles (GM). GTEs with and without contractions of the TA and GM muscles were selected and connected to a 4-channel ENG system designed to amplify ENGs by 100,000 times and to pass frequencies between 500 Hz and 10 kHz. In our study, 12 experiments were conducted on three Beagle dogs with a cuff implanted for up to 2 years. We present the results obtained in four experiments conducted on one animal. With the implanted leg mounted in a special electronic brace we applied extending forces to the ankle, rotating it by up to 37° according to the neutral position, eliciting torque to stretch the TA muscle. Only the ENG from a GTE eliciting maximum contraction of the TA muscle showed activities corresponding to the trajectory of the mechanical load of the muscle. Next, we dissected the calcanean tendon (CT) of the implanted leg and applied repetitive pull forces to the CT. Only the ENG from the GTE eliciting maximum contraction of the GM muscle was activated in correspondence to the trajectory of the mechanical load applied on the CT. The results suggest that the cuff, implanted chronically on the sciatic nerve, is useful to record ENGs of the afferent fibers from TA and GM muscles selectively and that the technique could be extended for human use in the field of rehabilitation for paralysis.