Abstract
1. A large amount of magnesium was necessary for the clearing response of actomyosin and its degree increased and its duration prolonged with increasing the concentrations of magnesium and ATP.
2. A small amount of magnesium was necessary for the process of the superprecipitation.
3. Calcium inhibited the clearing response depending on its concentration and by it accelerated the process of the superprecipitation.
4. The inhibitory effect of calcium on the clearing response was in linear relation to the logarithm of the concentration of calcium. This effect of calcium was to a large extent dependent on the conditions of the reaction.
5. Calcium, strontium or barium inhibited the clearing response. On the other hand, the addition of magnesium, manganese or bivalent iron led markedly to the clearing response. The shorter the ionic radius of bivalent cation was, the more remarkable the clearing response was.
6. In the presence of magnesium, other bivalent cations inhibited the clearing response and accelerated the process of the superprecipitation. The degree of these effects was dependent on the ionic radius of bivalent cation, and was maximum at 0.95×10-8cm.
