Abstract
Determination of the intracellular localization of acid phosphatase by use of differential centrifugation showed an even distribution of the enzyme activity among mitochondrial, microsomal and soluble fraction of the whole brain. Microsomal fractions from the adenohypophysis and anterior hypothalamus contained less portion of the total activity. It was found that the enzyme in the soluble fractions was more inhibited by formaldehyde and less by tartrate or fluoride as compared with that in other fractions. Acid phosphatase of the rat adenohypophysis was chromatographically separated into three and that of the anterior hypothalamus into two, major components of activity. Prolonged exposure of animals to moderate cold brought about an increase in per cent distribution of the first major component. From the similarity in the pattern of per cent inhibition by various inhibitors between of these chromatographic components and the fractions after differential centrifugation, a possibility was suggested that the acid phosphatase primarily related to the secretory activity may be separable as an independent peak in DEAESephadex column fractions.
