PHOTO-RELEASE OF OXYGEN FROM RHODOPSIN SOLUTION

Abstract

1. Oxygen uptake of rod outer segments was apparently inhibited following illumination.
2. When rhodopsin solution was illuminated, a photo-release of molecular oxygen from rhodopsin solution was observed, possibly leading to an inhibition of the oxygen uptake of rod outer segments.
3. Optimum pH for the release of molecular oxygen was 6.7-6.8.
4. Action spectrum for photo-release of molecular oxygen from rhodopsin solution was observed in a spectrum region between 450-560 mμ.
5. Light absorbed by interaction between π-electron of vitamin A aldehyde and opsin may contribute to the release of O₂ from rhodopsin solution; light absorbed by vitamin A aldehyde and opsin themselves was non-effective for the release of O₂.