Abstract
“Hybridization” of adult human and canine hemoglobins is greatly depressed by the deoxygenation, which suggests that the strength and/or the nature of the inter-chain linkages may undergo a reversible and profound alteration dependent upon the ligand binding. A non-linear relationship is observed between the extent of the “hybridization” and that of the oxygenation, which implies that the above stated alteration in the quaternary structure of hemoglobin proceeds in a non-straightforward manner with the ligand binding.
