Membrane Potential of Rat Urinary Bladder Epithelial Cells

Abstract

Rat urinary bladder epithelial cells exhibited a membrane potential of about -22 (apical) and -24 (basal) mV in normal Krebs solution. Blockade of Na⁺ entry through the apical membrane by Na⁺ depletion or administration of amiloride, or inactivation of the Na pump in the basal membrane by ouabain resulted in marked hyperpolarization of the epithelial cells. N-butyl-N-(4-hydroxybutyl) nitrosamine (BBN), a specific carcinogen, and saccharin, a potent enhancer of carcinogenesis, inhibited development of the Na⁺ channel and/or Na pump on the bladder epithelium. This physiology may contribute to the abnormal intracellular environment leading to bladder carcinoma.