Abstract
Seven dogs, anesthetized with evipan-sodium, were experimented on. The adrenal venous blood specimens were collected through the lumbar route and the adrenaline content of the specimens was estimated colorimetrically by means of the arseno-molybdic acid method of Bloor and Bullen.
On receiving acetylcholine in a dose of2mg. per kg. the adrenaline secretion of the adrenal gland was remarkably augmented and its mean value during90 seconds after the start of injection was estimated as0.41-0.97μg. per kg. perminute.
After a while, hexamethonium in a dose of0.25-1.0mg. per kg. was applied and then acetylcholine in the same dose as before was injected again. In all cases, except in one instance when hexamethonium was given in a dose of0.25 mg. per kg., the value after the2nd acetylcholine injection was noticeably small, 0.02-0.14μg. per kg. per minute being estimated there.
From these experimental results, it is deducible that the adrenaline-secretory effect of acetylcholine is inhibited by hexamethonium.
