ON THE ACCELERATION OF BLOOD CATALASE REACTION WITH SURFACE-ACTIVE AGENTS
1956 Volume 6 Pages 59-67
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1956 Volume 6 Pages 59-67
The effect of surface-active agents on the catalatic reaction by hemolysate was investigated by the method of thermal analysis of the reaction velocity and the relation between reaction velocity and surface tension has been considered. The results are summarized as follows:
1. The surface-active agents essentially accelerate the catalatic reaction by hemolysate with a certain range of their concentrations, whether or not they are cationic, or anionic, or nonionic.
2. The reaction type is mainly of the first order when the surface-active agent is added, and the maximum of the reaction velocity takes place at concentrations near to the critical micelle concentration giving the minimum on the surface tension-concentration curve.
3. The acceleration by surface active agent is due to the molecular adsorption of a surface-active agent to the blood catalase molecule.
4. The retardation of the reaction velocity comes out only when the concentration of the surface-active agent is very high as compared with the critical micelle concentration, owing to the accumulation of a surface-active agent.
Outline of this report was read before the32th general meeting of Japanese Physiological Society (1955).
