Abstract
The study was made of the effects of various agents (MgCl2, CaCl2, Napyrophosphate, ethylendiamine tetraacetate (EDTA), and salyrgan) on the solubility of actomyosin under the influence of low concentrations of KC1, at. PH 7.
The results obtained were as follows:
(1) In the presence of KC1 of physiological concentration at pH 7, each agent examined had dual precipitating action on actomyosin, as the concentration of the agent increases (“phenomenon of dual precipitation”).
(2) In the case of EDTA, the “phenomenon of dual precipitation” was slightly modified, and in the case of salyrgan, a third supplemental zone of precipitate appeared at a high concentration of salyrgan.
(3) The authors have pointed out that the “phenomenon of dual precipitation” is worthy of attention as one of the peculiar characteristics of actomyosin and as one important key-point to clarify the mechanism of superprecipitation or muscular contraction.
