FREE AMINO ACIDS IN THE CHOROID AND EFFECTS OF SOME METABOLITES ON THE RETINAL PIGMENT MIGRATION

Abstract

1. Determination of free amino acids in the choroid of the toad's eye was carried out by means of circular buffered paper chromatographic technique. From the visual comparison of the spots, the relative abundance of amino acids in the choroid was glutamic acid≥alanine>serine>aspartic acid.
2. A microscopic observation has been made on the retinal pigment migration of the frog's eye by means of intraocular injection method. Injection with aspartate into the dark-adapted eye, evoked an extreme migration of the retinal pigment, despite the fact that it was placed in the dark. Injection with either glutamate or oxalacetate, exhibited a partial pigment migration. Injections with another substances used in this experiment, had no effect on the pigment migration in the dark. When the dark-adapted frog was exposed to light after the injection with either NH₄Cl or α-alanine, the pigment migration was not evoked.