Japanese Journal of Phytopathology
Online ISSN : 1882-0484
Print ISSN : 0031-9473
ISSN-L : 0031-9473
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Detection of Acidovorax avenae subsp. citrulli from seed using LAMP method
H. OYAH. NAKAGAWAN. SAITOH. UEMATSUT. OHARA
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2008 Volume 74 Issue 4 Pages 304-310

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Abstract
Because detection methods for the causal agent of bacterial fruit blotch, Acidovorax avenae subsp. citrulli (Schaad, Sowell, Goth, Colwell, Webb, Willems, Goor, Thielemans, Gills, Kersters & DeLey), from seeds are time-consuming we evaluated a new assay using membrane filtration and loop-mediated isothermal amplification (LAMP). A LAMP primer set was designed for specific amplification of the hrpG-hrpX gene spacer region in A. avenae subsp. citrulli. The detection sensitivity of A. avenae subsp. citrulli of this procedure was approximately 103 cfu in 100 ml of water used to wash 1000 artificially infested seeds of either watermelon, wax gourd or melon. The complete assay took approximately 2 h. When naturally infected seeds were tested, A. avenae subsp. citrulli was detected from melon and watermelon seeds. This method is valuable for the rapid and precise detection assay of A. avenae subsp. citrulli on seeds.
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© 2008 The Phytopathological Society of Japan
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