Cloning of lipoxygenase cDNA from Actinidia arguta

Abstract

A 2658-bp full-length cDNA encoding a lipoxygenase (AaLOX1) was identified from Baby kiwi (Actinidia arguta). The deduced amino acid sequence of the gene is about 70% identical to plant liopxygenases (LOXs) of strawberry, potato and cucumber. The amino acid sequence possesses a PLAT motif and several of the typical LOX motifs. AaLOX1 is predicted to be located in the cytoplasm. AaLOX1 is inducible, and the transcripts accumulate in leaves within 0.5 hr after either wounding or inoculation with a pathogen or nonpathogen.