Abstract
Correlation analysis was performed between elicitor-responsive photon emission (ERPE) and hydrogen peroxide generation (HP) in suspension-cultured rice cells elicited with N-acetylchitohexaose. With elicitor concentrations from 0.03 to 1000 μM, ERPE and HP followed similar patterns in time-course studies. In addition, both ERPE and HP increased with increasing concentrations of N-acetylchitohexaose according to a natural logarithm function. On the basis of these results, we clarified that ERPE and HP have similar generation patterns. To analyze the quantitative relation between ERPE and HP, we measured both ERPE and HP fluctuations after altering concentrations of the elicitor or using the priming effect of a plant activator. The correlation coefficient between ERPE and HP was 0.95~0.97 (P<0.01) for the elicitor, and 0.90~0.93 (P<0.01) for the activator. Thus, we elucidated that ERPE is quantitatively correlated with HP.
