Simplified method to isolate Clavibacter michiganensis subsp. michiganensis using ImmunoStrip^TMCmm.

Abstract

A method to isolate Clavibacter michiganensis subsp. michiganensis (Cmm) using ImmunoStrip^TMCmm (IS), a commercial strip to diagnose bacterial canker on tomato (Solanum lycopersicum) caused by Cmm, was developed. A distinct positive antigen-antibody reaction line developed for suspensions of Cmm over 2×10⁵ cfu/ml. The Cmm-positive band on the IS was then cut from the strip, friction-ground in 0.1 ml sterilized distilled water, and a loopful of suspension was plated on potato semisynthetic agar for a fairly selective isolation of Cmm.