Abstract
An accessory food substance was proved to be essential to the growth of Bact. hyacinthi SMITH It was contained more in the bodies or staled solution of Bact. solanacearum and less in oryzanin; both readily being available to the organism. This substance was denatured by filtrating through filter paper and destroyed by heating at 100°C for 30 minutes in successive three days. It is soluble more in water than alcohol. From its nature the author is inclined to believe that it may be identical with vitamine D, a cleavage from vitamine B as shown by FUNK and DUBLIN.
The amount of this substance, necessary to the growth of the organism, is fixed; it being 3 per cent in beef bouillon when oryzanin employed as the source of this substance.
Its excessive amount had no noticeable influence on the growth of the organism, but its low amount limitted the growth of it as proportional to its content. In the case of the excessive supply of the substance, the final growth of the organism, in spite of its amount, was always the same, though slightly favourable growth was observed at the beginning. It may be attributable to the population density of the organism.
The intermittent growth was observed in the course of their culture, which seems to be comparable to ‘Hexenrings’ in fungus plate.
